植物生长发育调控研究组

拟南芥NF-Y核因子通过表观遗传调控整合植物的开花反应

开花作为从营养生长到生殖生长的过渡,是植物生命周期中最重要的发育阶段。植物激素调控研究组侯兴亮研究员通过与新加坡国立大学的合作研究,发现拟南芥NF-Y核因子复合物通过整合发育和环境信号,从表观遗传层面上调控开花时间。NF-Y是在真核生物中广泛存在的一类CCAAT结合转录因子,由三个不同的亚基组成,通过与其它因子相互作用来调控下游基因表达。在植物中,NF-Y参与调节植物包括逆境反应、胚胎和叶绿体发育、光合作用等重要生理过程。研究结果证实,NF-Y与光周期途径中的CONSTANS以及GA信号通路中的DELLA蛋白发生互作,直接调控了SOC1的转录。这一NF-Y复合物直接结合到SOC1基因启动子一个独特的顺式元件上,并通过招募H3K27去甲基化酶REF6影响SOC1 DNA的H3K27me3修饰。 该研究结果确立了植物NF-Y复合物作为重要的表观遗传相关因子,调控了植物对内在发育信号和环境的反应。相关研究成果发表在Nature Communications(Hou et al., 2014, 5: 4601)。

Nuclear factor Y-mediated epigenetic regulation orchestrates flowering responses of Arabidopsis

The transition from vegetative to reproductive development is crucial for reproductive success of flowering plants. Hou in Phytohormone Regulation Lab and his collaborators in NUS found that nuclear factor Y (NF-Y)-mediated epigenetic regulation orchestrates flowering responses to intrinsic and external clues. NF-Y, a type of CCAAT box-binding combinatorial transcription factors consisting of three subunits, is widely existed and conserved in eukaryotes. It is reported that NF-Y usually regulates downstream expression through mutual function with its cofactors. In plants, NF-Y has been shown to be involved in important growth and development processes including stress response, embryogenesis, chloroplast formation and photosynthesis etc. In this study, it demonstrates that NF-Y interacts with CONSTANS in the photoperiod pathway and DELLAs in the gibberellin pathway, to directly regulate the transcription of SOC1, a major floral pathway integrator. This NF-Y complex binds to a unique cis-element within the SOC1 promoter to modulate trimethylated H3K27 levels, partly through a H3K27 demethylase REF6. These findings establish NF-Y complexes as critical mediators of epigenetic marks that regulate the response to environmental or intrinsic signals in plants, which was published in Nature Communications(Hou et al., 2014, 5: 4601).

 

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